Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Repression of GUS activity in the roots of the line UBQ10:5`UTR-GUS/GFP-I. (A) Histochemical staining. Seedlings were germinated and grown for four days in 1/2 MS supplemented with 20 mM sucrose and transferred to 1/2 MS with the indicated sugar for 24 h. From left to right: no sucrose, 20 mM, 100 mM, 200 mM, 300 mM sucrose, 300 mM sorbitol. (B)Quantification of GUS activity relative to the protein concentration in shoots and roots. Seedlings were grown 5 days on horizontal 1/2 MS plates with 1% plant agar, supplemented with the indicated sucrose concentration in mM or 200 mM sorbitol (Sorb). GUS activity shown relative to the activity of seedlings grown without sucrose.