Supplemental Data

Files in this Data Supplement:

• Supplemental Data
• Supplemental Tables
• Supplemental Figure 1 - Analysis of individuals segregating the dTph1 insertion in the MADS box of UNS. The acrylamide gel shows PCR products amplified with UNS gene specific primers. Several plants are hemizygous for the insertion (lanes 1, 3, 4, and 7), whereas only plant #6 appears to be homozygous. The lower amplification product is the wild type UNS fragment, whereas the upper prominent band represents a UNS fragment containing the 284-bp dTph1 transposable element. The faint band, indicated by an arrow and slightly higher than the wild-type one, is due to somatic excision of the transposon. This was confirmed by direct sequencing of the PCR product, which revealed the presence of an 8-bp footprint left upon excision of the transposon. Since the footprint is responsible for a frameshift in the open reading frame, it is expected that no wild-type UNS protein is formed in plant #6. M, molecular marker lane; WT, wild type.