Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - In situ hybridization of APX2 mRNA in a transverse section of a fully expanded rax1-1 leaf (top left panel) of 18-d-old long day grown plants under low light conditions. The top right panel is wild-type (Col-0). The bottom left colored panel was prepared at a different time with the same probe and is of Col-0 subjected to a 10-fold excess light stress for 45 minutes. Note the hybridization is associated with the vasculature.
• Supplemental Figure 2 - Steady state levels of antioxidant defense gene transcripts in rax1-1 and wild-type plants under non-stress conditions. Note that the suffix A and B refers to both independent isolates of rax1-1 (see Results). This experiment was completed before the mapping was undertaken.
• Supplemental Figure 3 - Quantum efficiency of photosystem II in low light grown, excess light stressed, and post-stress wild-type, cad2-1, and rax1-1 in mesophyll and veinal tissue. For determination of φPSII, 6-week-old low light cultivated plants were subjected to 90-min EL stress (2000 +/- 200 μE). Prior to the stress treatment, after 80 and 90 min exposure and after 60 and 70 min of recovery in low light, each one fully expanded leave was harvested for fluorescence analysis with a Microscopy PAM and the manufacturer's software (Heinz Walz, Effeltrich, Germany). On each leaf, four measurements were taken from the lower side, two on the midvein, and two on mesophyll tissue. The measuring area was ~1 mm². Before application of a saturating pulse, the area of measurement was adapted to low light (37 μE blue light) for 90 sec. This time was usually sufficient to obtain a stable fluorescence signal Fs. φPSII was calculated as (F'm - Fs)/F'm (Baker et al., 2001). Data presented are means +/- SD. For each line, at least four plants were analyzed, and time points after stress and after recovery were pooled, yielding a total of at least 16 measurements per data point. Extremely high or low fluorescence values were discarded.
• Supplemental Table 1
• Supplemental Table 2