Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - T-DNA insertion characterization. (A) Southern blot using the T-DNA as a probe. (B) FISH. Centromeres (green) and T-DNA (red) are visualized in a pachytene nucleus in mpa1.
• Supplemental Figure 2 - mpa1 Metaphase I. One bivalent (II), six univalents (I) and four chromatids (1/2).
• Supplemental Figure 3 - Wild-type fertility and meiosis are restored after complementation of the mpa1 mutant. (A) Phenotype of siliques from a wild-type, an mpa1 mutant, and a complemented plant. Both silique size and the number of seed per silique were restored to normal in the complementation line. ([B] and [C]) Male meiosis in the complemented line. (B) Metaphase I. Five normal bivalents are visible (n = 50 cells). (C) Metaphase II. Two groups of five chromosomes. The scale bars represent 5 mm (A) and 10 μm ([B] and [C]).
• Supplemental Figure 4 - Alignment of the metallopeptidase domain of MPA1 and PSA homologs in O. sativa, H. sapiens, M. musculus, D. melanogaster, and C. elegans. Identical amino acids appear in red. Similar charged amino acids are in blue letters. Gaps are represented by a slash. Open boxes show the metallopeptidase zinc-binding signature, HEXXH(X)¹⁸E.
• Supplemental Figure 5 - Immunolocalization of MSH4 and MLH1 in wild-type and mpa1. ([A] to [C]) Immunolocalization of MSH4 (red) in wild-type. (A) Leptotene MSH4 is distributed as multiple foci on the chromatin, which decrease as prophase I progresses. (B) Zygotene. (C) Pachytene. (D) MSH4 zygotene in mpa1 the protein is accumulated in the cytoplasm. (E) Immunolocalization of MLH1 (red) and ASY1 (green). In wild-type at zygotene. MLH1 the 9-10 visible foci are distributed on the chromosomes axes. The scale bar represents 10 μm.
• Supplemental Table 1