Supplemental Data

Time-lapse movies of amyloplast and vacuolar membrane dynamics.

Files in this Data Supplement:

• Supplemental Figure 1 - The procedure of the preparation sample for observation using a vertical stage microscope.
• Supplemental Movie 2 - Amyloplast dynamics in the endodermal cell of an Arabidopsis inflorescence stem (wild-type background, pspt3-6, see method) before gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 3 - Amyloplast dynamics in the endodermal cell of an Arabidopsis inflorescence stem (pspt3-6) after gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 4 - Vacuolar membrane dynamics in the endodermal cell of an Arabidopsis inflorescence stem (wild-type background, psgt4-1, see method) before gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 5 - Vacuolar membrane dynamics in the endodermal cell of an Arabidopsis inflorescence stem (psgt4-1) after gravistimulation by reorientation. Each image was taken at 5-sec. intervals and 20 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 6 - Amyloplast dynamics in the endodermal cell of an Arabidopsis inflorescence stem (pspt3-6) treated with latrunculin B (2 μM) for 14 h, before gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 7 - Amyloplast dynamics in the endodermal cell of an Arabidopsis inflorescence stem (pspt3-6) treated with latrunculin B (2 μM) for 14 h, after gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 8 - Amyloplast dynamics in the endodermal cell of an Arabidopsis inflorescence stem (zig-1/sgr4-1 background, GFP marker was introduced by crossing with pspt3-6) before gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 9 - Amyloplast dynamics in the endodermal cell of an Arabidopsis inflorescence stem (zig-1/sgr4-1 background, GFP marker was introduced by crossing with pspt3-6) after gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).
• Supplemental Movie 10 - Vacuolar membrane dynamics in the endodermal cell of an Arabidopsis inflorescence stem (zig-1/sgr4-1, zig-1psgt1-1, see method) before gravistimulation by reorientation. Each image was taken at 10-sec. intervals and 10 frames of the time-lapse pictures were played every 1 sec. (x100 speed).