Supplemental Data

Files in this Data Supplement:

• Supplemental Data 1
• Supplemental Table 1
• Supplemental Table 2
• Supplemental Table 3
• Supplemental Figure 1 - Supplemental Figure 1. Meristems of 12 Day-Old cna-1 Mutants Are Larger than those of Wild-Type Plants. Inflorescence shoot apical meristems were collected from multiple wild-type Ler and cna-1 plants. Scanning electron micrographs of the smallest and largest meristem of each genotype are shown. See Table 1 for means and sample sizes. Bar = 50 μm. All images shown at same magnification. (A) Smallest wild-type Ler meristem (B) Largest wild-type Ler meristem (C) Smallestcna-1 meristem (D) Largest cna-1 meristem
• Supplemental Figure 2 - Supplemental Figure 2. The cna-1 Mutation Enhances Weak, Intermediate, and Strong Clv? Mutant Phenotypes. Scanning electron micrographs of 15 day-old inflorescence apices of clvsingle and clv cna-1 double mutant apices. (A) and (B), (C) and (D), and (E) and (F) are shown at the same magnification, respectively. Bars = 100μm in (A) and (D), and 200 μm in (F). (A) clv1-7 meristem (B) clv1-7 cna-1 meristem. (C) clv2-1 meristem (D) clv2-1 cna-1 meristem (E) clv1-4meristem (F) clv1-4 cna-1 meristem
• Supplemental Figure 3 - Supplemental Figure 3. Transcript Accumulation in Mutant Lines. For each allele used in this study, RT-PCR was used to detect the presence of transcripts. The locations of the mutations and primers used for RT-PCR are indicated for each gene. Arrows in T-DNA insertion rectangles indicate the likely locations of P35S promoter sequences. With the exception of clv3-2, clv3-2 cna-1, and clv3-2 cna-2, RNA was extracted from the aerial portions of two-week-old seedlings and reverse transcribed using a poly-dT primer. Inflorescence apices were used for RNA extraction from clv3-2, clv3-2 cna-1, and clv3-2 cna-2plants. After amplification, samples were electrophoresed on agarose gels and photographed. Primers for the β-ATPase gene were assayed for each RNA sample to control for quality and quantity of cDNA (Prigge and Wagner, 2001).
• Supplemental Figure 4 - Supplemental Figure 4. CNA is Expressed in Developing Vascular Elements, Flowers, and Ovules Sections of wild-type Ler ([A] to [E]), clv3-2 cna-1 (F) and clv3-2 (G) tissues were hybridized with anti-sense ([A] to [F]) and sense (G) dioxygenin-labeled CNA riboprobe. (A) Stage 4 to stage 7 flowers showing CNA signal diffusely throughout flower meristem and young organs. (B) Stage 9 and older flowers showing CNA signal strongest within developing stamens and carpels. (C) Ovules at early stages of initiation showing CNA signal. (D) Older ovules continue to express CAN. (E) CNA is most strongly expressed in developing vascular tissue. (F) cna-1 continues to be expressed in apices of clv3-2 cna-1 plants undergoing differentiation. (G) Sense controls show no signal.