Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Assessment of array results by RT-PCR expression studies for selected petal stage 3 DEF target genes. RT-PCR experiments were conducted as described in Figure 6.
• Supplemental Table 1 - List of differentially expressed ESTs shown in Figure 2. ESTs were selected showing in at least one of the four investigated wild-type petal conditions (P1, P2, P3, and P4) a >1.2-fold expression change compared with wild-type sepals. Intensity threshold: 175000000.
• Supplemental Table 2 - Coregulated subsets of ESTs differentially expressed between wild-type petals and sepals. (A) ESTs significantly regulated (>1.2-fold change) only in stage 1 wild-type petals (P1) compared with sepals. (B) ESTs showing a significant up-regulation (>1.2-fold change) in late wild-type petal stages (P3 and P4) compared with sepals. (C) ESTs significantly down-regulated (>1.2-fold change) in all investigated wild-type petal stages (P1-P4) compared with sepals.
• Supplemental Table 3 - List of differentially expressed genes regulated by DEF during def-101 petal stage 3 shown in Figure 5. Genes were selected that showed in at least one investigated condition (def-101, 24 and/or 72 h shift) a >1.2-fold change compared with the not-shifted control condition. Intensity threshold: 296800000.
• Supplemental Table 4 - List of temperature-sensitive ESTs detected in stage 3 wild-type petals. Listed are ESTs, showing in at least one investigated condition (wild type, 24 and/or 72 h shift) a >1.2-fold change compared with the not-shifted control condition. Intensity threshold: 120800000.
• Supplemental Table 5 - List of differentially expressed ESTs between petal stage 3 wild-type and def-101 plants. ESTs were selected showing in the investigated condition (def-101 P3) a >1.2-fold change compared with the control condition (wt P3). Intensity threshold: 121250000.
• Supplemental Table 6 - Pearson correlation coefficients of all macroarray experiments to assess filter quality, reproducibility of hybridization repetitions, and degree of differential expression between investigated conditions. (A)Coefficients for MCE (multi-conditional experiment) wild-type stages. (B) Coefficients for MCE stage 3 petal def-101 shifts. (C)Coefficients for MCE stage 3 petal wild-type shifts.
• Supplemental Table 7 - Primer sequences used for RT-PCR and in situ analyses. Reverse primers for in situ template production contain T3 RNA polymerase binding sites.