Supplemental Data

Files in this Data Supplement:

• Supplemental Video 1 - Video of moving granules. Small motile granules of GFP-TGB2 expressed from a TMV vector moving in the ER network.
• Supplemental Figure 1 - TGB2 and TGB3 expression from TMV vectors in N. benthamiana. (A) Following manual inoculation, TMV-expressed GFP-TGB3 labels the ER network and small motile bodies associated with the network. This image shows cells at the leading edge of the infection front, 2 dpi. Bar = 20 μm. (B) Similar to that shown in (A), TMV-expressed GFP-TGB2 also labels the ER network and small motile bodies associated with the network following manual inoculation. This image shows cells at the leading edge of the infection front, 2 dpi dpi. Bar = 20 μm. (C) Two days after bombardment, GFP-TGB3 expressed from a TMV vector with a defective movement protein (TMV.ΔMP.GFP-TGB3) labels the same structures as when expressed from a vector containing a functional MP (c.f. [A]). Bar = 20 μm. (D) Two days after bombardment, GFP-TGB2 expressed from a TMV vector with a defective movement protein (TMV.ΔMP.GFP-TGB2) labels the same structures as when expressed from a vector containing a functional MP (c.f. [B]). Bar = 20 μm. All images are reconstructions of the cell using Z stacking.
• Supplemental Figure 2 - The small motile structures labelled by mRFP-TGB2 do not co-localize with Golgi bodies. An epidermal cell of transgenic A. thaliana expressing a GFP fusion to sialyltransferase shows GFP-labelled Golgi. When bombarded with P_35S-mRFP-TGB2, there is no co-localization of the small motile structures labelled by mRFP-TGB2 (arrow) with the Golgi (arrowhead). 2 dbp. Image is a reconstruction of the cell using Z stacking. Bar = 5 μm.