Large Intraspecific Haplotype Variability at the Rph7 Locus Results from Rapid and Recent Divergence in the Barley Genome
Plant Cell Scherrer et al.
17: 361
Supplemental Figure 1
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Dot plot comparison of the sequences from Morex and Cebada Capa. 126.6 kb of sequence from Morex (AF521177, position 85,000 to 211,664) was aligned with 12,258 bp (AY642925) and 184,426 bp (AY642926) from Cebada Capa. The lines above each axis represent the entire region in each cultivar. Genes are indicated as black boxes and BARE-1 LTR retrotransposons are in grey. Seven conserved regions (CR) are indicated as lines delimited by arrowheads. The interrupted line represents the ~30 kb of unassembled BARE-1 sequence in Cebada Capa.
Supplemental Figure 2
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Schematic representation of the comparison between the Hvgad1-Hvpg1 intergenic regions (A) and the Hvpg1 and Hv283 interval (B) in Morex and Cebada Capa. The seven conserved regions (CR) are indicated with light blue areas. The size of the intergenic regions in each of the two sequences is indicated between double arrowheads. The partially sequenced 30 kb region containing several BARE-1 elements is shown between two vertical dotted lines. The rearranged duplications of 21 kb and 9 kb in the CR2-CR3 interval in Cebada Capa are shown below the annotation. Genes are indicated as black boxes with their name above them and their transcriptional orientation is shown with an arrow. Repetitive elements are drawn as boxes with the color code indicated in the legend. Complete sequence annotations are given under the accession numbers AF521177 for Morex and AF642925-26 for Cebada Capa.
Supplemental Figure 3
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PCR amplification on 17 barley cultivars representative for the different haplotypes found at three independent loci. Line 1: Bowman, 2: Bowman5*/Clipper BC67, 3: Triumph, 4: Bowman*/PI531849, 5: Egypt, 6: Sundance, 7: Tunisian, 8: Elisa, 9: Ellinor, 10: Franka, 11: Hanka, 12: Hor4445, 13: JCJ-188, 14: L94, 15: Quinn, 16: Michka, 17: Pallas.