Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplementary Figure 1. Nucleotide sequence of RIN13 (At2g20310.1) cDNA. Nucleotides in grey correspond to the 196 nucleotide 5' upstream region of RIN13 and the 176 nucleotide downstream region. Introns (size in bp) are present at positions 330 (375) and 417 (88) in the coding sequence. The lowercase underlined "a" at position -1 was mutated to "c" to create an Nco-1 restriction endonuclease site at the initiating methionine (see Material and Methods). The grey arrow represents the insertion site of the T-DNA in SALK_001145.
• Supplemental Figure 2 - Supplementary Fig. 2 The enhanced resistance phenotype of RIN13s is suppressed in the Atrar1 background. Homozygous RIN13s lines were generated in the Atrar1-28 background. Bacterial growth in RIN13s Atrar1-28 were compared to Atrar1-28 plants following challenge with DC3000(avrRpm1) (white and dark grey respectively) or DC3000 (light grey and black respectively). No enhanced suppression of bacterial growth was evident, suggesting RAR1 functions downstream of RIN13 elicitation.
• Supplemental Figure 3 - Supplementary Fig. 3 Biophoton generation in RIN13s leaves challenged with DC3000(avrRpm1) occurs co-incident with DC3000 induced biophotons. Upper panel: Bright field image of wild type Col-5 plant and RIN13s line challenged as indicated. Centre panel: Bioluminescence induced by the AvrRpm1/RPM1 interaction occurs only in wild type plants (photons integrated over 1 h). Lower panel: Weak biophoton generation is evident in both DC3000(avrRpm1) and DC3000 challenged leaves of RIN13s plants, 2-4 h prior to visible leaf wilting. All inoculations were at OD₆₀₀ 0.05.