Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. FASTA file of Clustal W 1.8 alignment and graphical alignment of MYB amino acid sequences.
• Supplemental Table 1 - Supplemental Table 1. Nucleotide sequence ofODO1and its deduced amino acid sequence. The longer 3' UTR is underlined.
• Supplemental Figure 2 - Supplemental Figure 2. Genomic DNA gel blot analysis indicates the presence of a single ODO1 copy in P. hybrida Mitchell.Genomic DNA of Mitchell was digested with EcoRI or EcoRV and separated on a 0.8% agarose-TBE gel prior to blotting. The C-terminal region used for the RNAi construct (see Materials and Methods) was employed as a probe. The size of the hybridizing bands is indicated in base pairs.
• Supplemental Figure 3 - Supplemental Figure 3. Suppression of ODO1 expression reduces benzenoid synthesis. Typical GC-TOF-MS chromatograms obtained with headspace-SPME of petal extracts from Mitchell and RNAi line 3. Petals were ground in saturated CaCl (for method see Verdonk et al., 2003). The combined current for ions 92, 105, 108, 136 and 164 is shown.
• Supplemental Figure 4 - Supplemental Figure 4. Emission of benzenoids is reduced in the F1 progeny of a cross between RNAi line 35 and V26 compared to a cross between Mitchell and V26. Typical and representative GC-TOF-MS chromatograms of volatile benzenoids emitted by Mitchell, V26, Mitchell x V26 and RNAi-line 35 x V26.
• Supplemental Figure 5 - Supplemental Figure 5. Petals of a cross between W115 and V26 with normal benzenoid emission levels and between RNAi line 35 and V26 with reduced benzenoid emission levels.
• Supplemental Figure 6 - Supplemental Figure 6. PAL1 and EPSPS are already expressed early during flower development.RNA gel blot analysis for ODO1, PAL1 and EPSPS during flower development. Hybridization with FBP1 is shown to indicate the loading of the gel.
• Supplemental Table 2 - Supplemental Table 2. Raw data of microarray experiments
• Supplemental Table 3 - Supplemental Table 3. Processed data of microarray experiments.