Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. Alignment of ATP-PRT cDNA Sequences Used in Phylogenetic Analysis. Abbreviations are as follows: A.les, Alyssum lesbiacum; A.mont, Alyssum montanum; A.thal, Arabidopsis thaliana; T. goes, Thlaspi goesingense; O.sativa, Oryza sativa; Z.mays; Zea mays; T.aestivum; Triticum aestivum; H.vulgare, Hordeum vulgare; M.trunc, Medicago truncatula; L.escul, Lycopersicon esculentum; S.tuber, Solanum tuberosum; P.patens, Physcomitrella patens.
• Supplemental Figure 2 - Supplemental Figure 2. Concentrations of Amino Acids Other than His are Generally Unaltered in Rosette Tissue of 35S:PRT2 Transgenic Plants. Free amino acid concentrations (relative to those in pBI121 empty-vector control plants) are shown for six 35S:PRT2 transgenic lines: two each with low transgene expression (4.4 and 18.2), intermediate expression (10.2 and 24.2), and high expression (21.2 and 30.5). The dotted line indicates a ratio of one (no difference to control plants). Amino acids are shown in order of decreasing abundance in pBI121 control plants. Three independent analyses were made per line, each consisting of two pooled plants, i.e. six plants were sampled per line. As a representative example of variation between samples, standard errors averaged 6% of the mean values for absolute His concentrations.
• Supplemental Table 1 - Supplemental Table 1. Percent Sequence Identity at the Nucleotide Level between ATP-PRT cDNA Homologues in the Brassicaceae Species abbreviations are as follows: Al = Alyssum lesbiacum; Am = Alyssum montanum; At = Arabidopsis thaliana; Tg = Thlaspi goesingense.
• Supplemental Figure 3 - Supplemental Figure 3. Ni Tolerance of 35S:PRT2 Transgenic Arabidopsis Plants Compared with Control Lines as Evidenced by Lack of Visual Toxicity Symptoms. Plants were precultivated after germination for 2 weeks on agar plates and were then transferred to hydroponic culture for a further 3 weeks on nutrient solution either without supplemental Ni (− Ni; left-hand panels) or with added 30 μM Ni (+ Ni; right-hand panels). From top to bottom, rows show wild type Col-0 plants (WT), empty-vector control plants (pBI121), and 35S:PRT2 transgenic lines 1.1, 22.1, 30.5, and 21.2. Further details are provided in Methods. WT and pBI121 control plants show pronounced metal-induced leaf necrosis following growth on 30 μM.