Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Figure S1. Immunoblot analysis of total protein extracts from wild type plants (lanes 1-3) and 35S:atTIC110His-induced atTIC110 silenced plants exhibiting a pale phenotype (lane 4). Serial dilutions of protein extracts from wild type and 35S:atTIC110His transformed plants were resolved by SDS-PAGE and immunoblotted with antisera corresponding to the proteins indicated to the left of the figure. The dilutions were chosen to give linear chemilumenescence signals with increasing protein levels. The signals from the chloroplast antisera were normalized to the corresponding actin signal for comparison. Lanes 2 and 4 were chosen for presentation in figure 3C because the actin signals were most similar.
• Supplemental Figure 2 - Figure S2. Accumulation of plastid proteins in atTic110NHis and atTic110CHis plants. Serial dilutions of total protein extracts from wild type, atTic110NHis and atTic110CHis plants were resolved by SDS-PAGE and immunoblotted with the indicated antibodies.The dilutions were chosen to give linear chemilumenescence signals with increasing protein levels. The signals from the chloroplast antisera were normalized to the corresponding actin signal for quantitation. (A) Lanes 3, 5 and 8 are shown in figure 6A and were chosen for the quantitative analysis shown in figure 6B because the levels of their actin immunoblot signals were most similar. (B) Lanes 2, 5 and 7 are shown in figure 9A and were chosen for the quantitative analysis shown in figure 9B because the levels of their actin immunoblot signals were most similar.