Supplemental Figure 1
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Figure S1. Multiple Sequence Alignment of the Arabidopsis CUL3 Proteins together with Human and C.elegans Orthologs.
The sequence alignment was made using the Clustal X (v1.83) software (Thompson et al., 1997). The ‘Gonnet’ protein weight matrices (Gonnet 40, 80, 120, 160, 250 and 350 matrices; Gonnet et al., 1992) were selected, and the gap opening and extension parameters were 10 and 0.2, respectively. Alignment of the amino acid sequences of AtCUL3A, AtCUL3B, CUL3 from H. sapiens (HsCUL3; Du et al., 1998) and C. elegans (CeCUL3; Kipreos et al., 1996). The numbers along the right of this figure indicate amino acid positions.
Du, M., Sansores-Garcia, L., Zu, Z., and Wu, K.K. (1998). Cloning and expression analysis of a novel salicylate suppressible gene, Hs-CUL-3, a member of cullin/Cdc53 family. J. Biol. Chem. 273, 24289-24292.
Kipreos, E.T., Lander, L.E., Wing, J.P., He, W.W., and Hedgecock, E.M. (1996). Cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85, 829-839.