Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Figure S1. cDNA sequence alignment of LnCENH3 and CENH3s from other plant species and canonical histone H3 from rice. Conserved nucleotide positions are indicated by asterisks above the nucleotides. Red boxes indicate the primer regions used in this study.
• Supplemental Figure 2 - Figure S2. Immunostaining of L. nivea using anti-LnCENH3 antibodies. (a) Low-magnification microscopic view containing metaphase (arrow), prophase (arrowhead) and interphase cells. LnCENH3 immunosignals and DAPI-stained L. nivea chromosomes are visualized as red and blue, respectively. (c, f, i and l) LnCENH3 immunosignals, (b, e, h and k) DAPI-stained L. nivea metaphase chromosomes, and (d, g, j and m) merged images of b and c, e and f, h and i, and k and m, respectively. Arrows in h-m indicate chromosomes viewed end-on from the telomere. Bar, 5 μm.