Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. Pro_35S:MIR160c and arf10-2 arf16-2 Plants Are Defective in Root Gravitropism. The 4-day-old vertical growing seedlings were turn 90°. Root tip positions, before (left column) and 12 hours after (right column) rotation, were scored. The length of each bar represents the percentage of seedlings showing direction of root growth within that sector.
• Supplemental Figure 2 - Supplemental Figure 2. Induction of ARF10 and 16 Transcription by Auxin. (A) RNA gel blot of ARF16 and miR160 expression in phytohormone-treated seedlings. The 7-day-old seedlings were treated with phytohormones in 1/2 MS liquid medium for 5 hours. 1. mock ; 2. indole-3-acetic acid (IAA, 50 μM); 3. abscisic acid (ABA, 50 μM); 4. 6-benzyl aminopurine (6-BA, 50 μM); 5. 1-aminocyclopropane-1-carboxylate (ACC, 50 μM); 6. brassinolide (BL, 10 nM); 7. gibberellic acid (GA, 50 μM). Arrow indicates the full-length mRNA, and asterisk indicates cleaved products. (B) Induction of ARF10 and 16 expression by different concentrations of auxin, analyzed by real-time RT-PCR. The relative amount of transcripts of each gene was quantified with respect to the Arabidopsis Actin2 transcript level. The 7-day-old seedlings were treated with IAA in 1/2 MS liquid medium for 5 hours; bars and error bars stand for means and SD, respectively. (C) Time course examination of induced expression of ARF10 and 16 genes by auxin, analyzed by real-time RT-PCR. The relative amount of transcripts of each gene was quantified with respect to the Arabidopsis Actin2 transcript level. The 7-day-old seedlings were treated with 50 μM IAA in 1/2 MS liquid medium. Plant materials were collected at the time interval as indicated; bars and error bars stand for means and SD, respectively.