Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. BP expression pattern in as2 and rdr6 as2 leaves. GUS staining was visible in as2-101 leaves (A and C) and was extended in rdr6-3 as2-101 leaves (B and D). The specimens in (A) and (B) were analyzed using a Zeiss Axioskop light microscope and those in (C) and (D) were viewed using an Olympus SZH-ILLB dissecting microscope. The leaves analyzed were from different as2 and rdr6 as2 plants, which were collected in the same F2 population of a cross between a plant carrying the BP::GUS fusion (Col) and an rdr6-3 as2-101 plant (Ler). Note that GUS staining in the as2 cotyledons was very weak or undetectable, but appears strongly at the distal end of the rdr6 as2 cotyledons.
• Supplemental Figure 2 - Supplemental Figure 2. miRNA filter hybridizations. This experiment was repeated twice using different transgenic lines, as shown in the upper and lower panels, respectively. Lengths of RNA fragments in nucleotide (nt) and probes used in hybridizations are indicated on the left and right of each hybridization image, respectively. RNA blots were first probed by miRNAs, and the same filters were then analyzed by 5S RNA probe.