Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. Stability of E2FB protein is positively regulated by synthetic auxin 2,4-D both in BY-2 and in Arabidopsis cells. Western blots of samples at 3, 4, and 5 days after subculturing in auxin (+2,4-D) and auxin-free (-2,4-D) media from E2FB/DPA (line 11), double-transformed transgenic BY-2 cells with anti-E2FB and with anti-myc antibodies to detect HA-tagged E2FB and Myc-tagged DPA protein levels, respectively. Arrows indicate the three forms of E2FB. Nonspecific protein bands cross reacted with the c-myc antibody were used as a loading control (A). Western blots of HA-E2FB or Myc-DPA transfected Arabidopsis protoplasts with anti-E2FB or anti-myc antibodies. The transfected protoplasts were maintained for 24 h in the presence or the absence of synthetic auxin 2,4-D (1 μM) before harvesting. Arrows indicate the three forms of E2FB (B). Western blots of Myc-E2FA or Myc-E2FB transfected Arabidopsis protoplasts with myc-antibody to show differences in their mobility (C).