Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. Subcellular localisation and FRET interaction studies of EDS1.
  (A) Arabidopsis pad4-1/sag101-1 leaves were transfected with EDS1-YFP via particle bombardment and analysed by confocal laser scanning microscopy. The image shows a 3D reconstruction from individual image stacks. N: Nucleus.
  (B) FRET-APB analysis of EDS1-CFP and PAD4-YFP. Arabidopsis eds1-1/pad4-5 leaves were co-transfected via particle bombardment and FRET-APB carried out 24 h after transfection. Mean FRET-efficiencies ± SD from at least 15 individual sample sites are shown. Mean FRET-efficiencies do not significantly differ from random FRET signals obtained for controls in Figure 8C and D.