Supplemental Data

Files in this Data Supplement:

• Supplemental Table 2 - Supplemental Table 2. List of the Primers Used for Semi-Quantitative RT-PCR Analyses.
• Supplemental Figure 1 - Supplemental Figure 1. Comparison between the udt1-1 and Wild-Type Meiocytes during Early Tapetum Development. The cross sections of wild-type ([A] to [C]) and udt1-1 ([D] to [F]) anthers at pre-meiotic stage ([A] and [D]), dyad stage ([B] and [E]), and young-microspore stage ([C] and [F]). MC, meiocytes; DMC, degenerated meiocytes; N, nucleus; D, dyads; C, callose wall; YP, young microspore; and WT, wild type. Scale bars = 2 μm.
• Supplemental Figure 2 - Supplemental Figure 2. DAPI Staining for Wild-Type and udt1-1 Anthers. (A) DAPI staining for wild type anther at meiosis. (B) DAPI staining for wild type anther at tetrad stage. (C) DAPI staining forudt1-1 anther at meiosis. (D) DAPI staining for udt1-1 anther at tetrad stage. Ep, epidermis; En, endothecium; M, middle layer; T, tapetal layer; Td, tetrads; WT, wild type; MC, meiocyte; and DMC, degenerated meiocytes. White arrow heads point to nuclei. All cells in anther walls have nuclei in the shape of circles. Scale bar= 10 μm.
• Supplemental Figure 3 - Supplemental Figure 3. Consistent Relationships between Microarray Analyses. Normalized log ratios of spots from a set and its dye-swap counterpart are shown in x- and y- axes, respectively. (A) Consistent relationship between udt1-1 [WT (cys3)_udt1-1(cys5)] and udt1-1_swapping 1 [WT(cys5)_udt1-1(cys3)_dye]. (B) Consistent relationship between udt1-1 [WT (cys3)_udt1-1(cys5)] and udt1-1_swapping 2 [WT(cys5)_udt1-1(cys3)_dye]. (C) Consistent relationship between M [Palea/lemma (cys3)_meiosis anther (cys5)] and M_dye swapping [Palea/lemma (cys5)_meiosis anther (cys3)]. (D) Consistent relationship between Y [Palea/lemma (cys3)_young microspore (cys5)] and Y_dye swapping [Palea/lemma (cys5)_young microspore (cys3)]. (E) Consistent relationship between V [Palea/lemma (cys3)_vacuolated pollen (cys5)] and V_dye swapping [Palea/lemmas (cys5)_vacuolated pollen (cys3)]. (F)Consistent relationship between P [Palea/lemma (cys3)_pollen mitosis (cys5)] and P_dye swapping [Palea/lemma (cys5)_pollen mitosis (cys3)]. Pl, wild type palea/lemmas; M, wild type anthers at meiosis stage; Y, wild type anthers at young-microspore stage; V, wild type anthers at vacuolated-pollen stage ; P, wild type anthers at pollen-mitosis stage.
• Supplemental Table 1 - Supplemental Table 1. Full List and Spot Intensities of All Genes that Are Up- or Down-Regulated at least Two-Fold in the udt1-1 Anthers during Early Tapetum Development. Data Sheet 1. Full list of all genes that are up- or down-regulated at least two-fold in the udt1-1 anthers during early tapetum development. Spot_ID are the names of oligomers selected by microarray. TIGR_CDS are putative coding sequences predicted by TIGR. KOME_cDNA are full-length cDNAs provided by KOME (http://cdna01.dna.affrc.go.jp/cDNA/). CHR_locus (http://www.tigr.org/tdb/e2k1/osa1/tigr_gene_nomenclature.shtml) is the chromosomal locus of genes that are up- or down-regulated at least two-fold in the udt1-1 anthers during meiosis to young-microspore stages. We selected 1225 Cys5 and Cys3 significant spots. Average values (AVR) and standard deviation (STD) are from three (udt1-1) or two (M, Y, V, and P) replicated experiments. The values are log ratios with base 2 udt1-1, the udt1-1/wild-type anthers at meiosis to young-microspore stages; M, meiosis stage anthers/palea-lemma; Y, young-microspore stage anthers/palea-lemma; V, vacuolated-pollen stage anthers/palea-lemma; and P, pollen-mitosis stage antehrs/palea-lemma. Data sheet 2 lists the Cys3 and Cys5 spot intensities for all the experiments.