Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1: RT-PCR analysis of iaaM and iaaL expression in effector lines. Homozygous T3 seedlings from EF GFP:GUS line #15, EF iaaM line #20 and EF iaaL line #11 were subjected to qualitative RT-PCR analysis (as in Weijers et al., 2001b) using primers specific for iaaM, iaaL or ROC7 (as an mRNA control). The same PCR reactions were performed on the EF plasmids. Note that iaaM and iaaL primers amplify a fragment from the respective plasmids, but not from the cDNAs derived from the corresponding plant lines.
• Supplemental Figure 2 - Supplemental Figure 2: Phenotypes of six week-old flowering plants expressing iaaM or iaaL transgenes. F1 progeny of crosses between ACT proLTP1 line #8 and EF iaaM line #20 or EF iaaL line #11. Note that the proLTP1>>iaaM plant is not branched whereas the proLTP1>>iaaL plant is more branched than the wild-type. Magnification is the same in all panels.
• Supplemental Figure 3 - Supplemental Figure 3: DR5rev-GFP activity in wild-type and iaaL-expressing embryos. Wild-type (left) or proRPS5A>>iaaL embryos carrying the DR5rev-GFP transgene, were cultured for 3 days in control medium (upper row), or in medium containing 1 mM lysine (lower panel). Note that DR5rev-GFP activity in proRPS5A>>iaaL embryos is unchanged relative to the wild-type, both with and without lysine.