Supplemental Data

Files in this Data Supplement:

• Supplemental Table 2
• Supplemental Table 3
• Supplemental Figure 1 - Supplemental Figure 1. Amino acid sequence comparisons and functional regions of CDPK proteins. The M. truncatula CDPK1 amino acid sequence derived from a cDNA (AY821654) was compared to the most similar presumptive CDPKs from tobacco (AAL30819), Arabidopsis (AAQ56823) and rice (AAF23901). The predicted protein kinase catalytic domain (hmmpfam (HMMRE2.1.1)) is shown in red. The serine/threonine protein kinase active-site signature sequence is underlined. The four regions predicted to be members of the EF-hand family (hmmpfam (HMM2.1.1.)) are shown in light blue with the embedded EF-hand Ca²⁺-binding domain (prosite PDOC00018) shown in dark blue. Dots (.) represent amino acid identities, dashes (-) represent gaps to obtain maximal alignment of the sequences.
• Supplemental Figure 2 - Supplemental Figure 2. CDPK1 gene structure. (A) Genomic sequence revealed that CDPK1 consists of 12 exons that encode a 560 amino acids protein. Location of DNA fragment used for PRO_CDPK1-GUS construct, RNAi constructs and for DNA gel probe are indicated. (B) DNA gel blot analysis of CDPK1 suggests that the gene is present as a single copy gene in M. truncatula genome. Ten mg of genomic DNA, cleaved with the indicated restriction enzyme, were used for each lane and probed with DNA probe indicated in (A). The PCR fragment (1.48 kb), amplified with primers 5’-GGGAAATTGTTGGGTCATGGA-3’ and 5’ CACAACCTGCAGGAGATC, was used as a probe. Two fragments containing CDPK1 genomic sequences hybridized to the DNA gel blot probe because of internal EcoRI and HindIII restriction sites.
• Supplemental Figure 3 - Supplemental Figure 3. Characteristics of EIN2i roots.(A) Control (WT) and EIN2i roots grown on BNM agar media. (B) EIN2i roots grown on BNM agar media inoculated with S. meliloti (16 dpi). (C) Composite plants with transgenic roots grown for 17 days after inoculation with A. rhizogenes grown on Fahraeus-agar medium supplemented with 27 mg/l of kanamycin.
• Supplemental Table 1