Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. Digestion of Leaf TAG and soy oil TAG with pancreatic lipase. Neutral lipid TLC was used as described under Methods. Lipids were visualized by iodine staining. TAG, triacylglycerol; FA, fatty acids; DAG, diacylglycerol; MAG, monacylglycerol; O, origin.
• Supplemental Figure 2 - Supplemental Figure 2. Phosphatidate phosphatase activity in isolated chloroplasts of the tgd1-1 mutant and the wild type.
• Supplemental Figure 3 - Supplemental Figure 3. Sodium chloride and sodium carbonate extraction of isolated pea chloroplast total membranes following import of MGD1. Intact chloroplasts were recovered, after 30 minute import assays, by centrifugation through a 40% Percoll cushion and fractionated into a total membrane and a supernatant fraction. The total membrane fraction (P) was subsequently extracted with either buffer (-), sodium chloride (NaCl) or sodium carbonate (Na2CO3). After extraction, all samples were centrifuged and separated into extracted membrane fractions (P) lanes 1, 3, and 5 and extracted soluble fractions (S), lanes 2, 4 and 6. All fractions were analyzed by SDS-PAGE and fluorography. TP, represents 10% of Translation product added to an import assay. pMGD1, precursor of MGD1; mMGD1, mature processed form of MGD1; tp110-110N, N-terminal fragment of TIC110 with transit peptide; 110N, N-terminal fragment of TIC110, after loss of transit peptide.
• Supplemental Figure 4 - Supplemental Figure 4. GFP-Immunoblot of leaf extracts of the wild type (WT) or the strongest TGD1-GFP expressing transgenic line (WT/TGD1-GFP). A monoclonal antibody against GFP was used. Equal amounts of protein were loaded (30 μg).