Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1: Alignment of putative proteins coded by FT-like genes from Arabidopsis (FT), Rice (HD3a) and Tomato (SP3D). Identity is in black and similarity in light blue. The SP3D protein is 75% identical to FT (134 amino acids of 178), and 86% similar. By comparison, the functional homologue of FT in rice, Hd3A is different from FT in 50 amino acids, yet retains early flowering activity in Arabidopsis (Kojima et al., 2002).
• Supplemental Figure 2 - Supplemental Figure 2: Relative expression of TFT in different Arabidopsis transgenic lines overexpressing the tomato SP3D/ TFT gene. TFT transcript levels in three homozygous lines (Pro35S:TFT # 4, 7 and 9; Figure 1D) were measured by RTPCR and real-time RT-PCR, (relative to β-tubulin). A. Agarose gel showing results from RTPCR on RNA samples from different lines using primers specific for TFT and β-tubulin. B. Results from Quantitative Real-time RT-PCR (see methods) using a Taqman probe for TFT. Expression is relative to β-tubulin (AT5G62690). All three lines expressed high levels with line Pro_35S:TFT#7 being slightly higher and this line was used for further genetic analysis.
• Supplemental Figure 3 - Supplemental Figure 3: Target gene expression in Pro_35S:TFT#3-7 plants. Expression was measured by quantitative Real-time RT-PCR (see methods). Expression of each gene was calculated relative to β-TUBULIN (AT5G62690). A. Comparison of 6 day old seedlings from different genotypes grown in blue long days (A3 in table 1). B. Expression in leaves of 30 day old wt and Pro35S:TFT#3 plants grown under short days (A4). C. Expression in leaves of 30 day old Pro_35S:TFT#3 plants grown under curling (A4) or non-curling (A1) conditions. D.Expression in leaves of 30 day old Pro_35S:TFT#3 plants with or without a mutation in FD. Plants were grown in non-curling long day conditions (A1). Each experiment represents one biological repeat and more than four technical repeats. Similar results were obtained with the Pro_35S: FT construct (figure 2).
• Supplemental Table 1 - Supplemental Table 1: Flowering time of Pro_35S:FT plants in different environmental conditions.
• Supplemental Table 2 - Supplemental Table 2. Leaf Curling and Flowering time of a Pro_35S:TFT#7 X sep3-2 segragating F2 population.