Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - The root phenotype of P_E7:axr2 transformant. (A) The control (Cont, P_E7:GFP) root. (B) and (C) Roots from P_E7:axr2 transformant. The dominant mutant gene (axr2) of AXR2/IAA7 was expressed in the root hair cell using P_E7. The P_E7:axr2 transformant root developed no root hairs. Bars=100 mm.
• Supplemental Figure 2 - Effects of chemicals on the restoration of root hair growth in P_E7:PID-GFP lines. Randomly chosen four independent lines (T2) of P_E7:PID-GFP (PIDox1∼4) were compared with the P_E7:PID-GFP control line (Cont). The legend for marks is the same in A through F. Bars indicate standard errors (n=48∼108 for root hair length and 10∼20 for root hair number). The x-axes are not scaled.
• Supplemental Figure 3 - Effects of chemicals on the restoration of root hair growth in P_E7:PIN3-GFP lines. Randomly chosen four independent lines (T2) of P_E7:PIN3-GFP (PIN3ox1∼4) were compared with the P_E7:GFP control line (Cont). The legend for marks is the same in A through F. Bars indicate standard errors (n=40∼96 for root hair length and 5~13 for root hair number). The x-axes are not scaled.
• Supplemental Figure 4 - Expression dosage unlikely affects the distribution pattern of mPID. (A to C) Confocal microscopy images of P_E7:mPID-GFP transformants expressing mutated PID (mPID)-GFP in the hair cell. mPID harbors a point mutation in its ATP-binding domain. A weakly expressing line (A) also showed the similar dispersed pattern of mPID as in strong lines (B and C). Bar=30 μm for all.
• Supplemental Table 1 - Expected sizes of PCR products in RT-PCR for PINs and PID