Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1.Alignment of the cyclin boxes of characterized G1 cyclins from fungi. Ustilago maydis Cln1 contained one cyclin box near the amino terminus that shared 73-30% of sequence similarity to those of other characterized G1 cyclins from fungi. These fungal G1 cyclins fall in two different classes, which reflects the presence or absence of an extra 37-21 amino acid stretch in the cyclin box. U. maydis Cln1 is grouped along with S. cerevisiae Cln3, S. pombe Puc1 and the C. albicans Cln1 and Cln3, all of them lacking the aforementioned amino acid stretch. Identical positions are shaded.
• Supplemental Figure 2 - Supplemental Figure 2. Formation of appressoria by wild-type and Δcln1 solopathogenic strains. Corn leaves infected with SG200 and SONU130 (SG200Δcln1) cells were sampled 1 day after infection and fungal structures were visualized on the leaf surface by calcofluor staining as described (Brachmann et al., 2003; EMBO J. 9, 2199-2210 ). No differences could be seen between infections by wild-type and infections by mutant cells. Bar, 10 μm.
• Supplemental Figure 3 - Supplemental Figure 3. Cln1 is not required for hyperpolarized growth induced by the mating program. (A) SONU8 cells, carrying an activated allele of the Fuz7 MAPKK, fuz7^DD (Müller et al., 2003) under the crg1 promoter., and its Δcln1 derivate UMN8 were grown in repressive (CMD) or inductive (CMA) conditions for fuz7^DD expression. Note that both mutant and wild-type cells were able to produce filaments. Bar, 20 μm. (B) AB33 cells, containing b compatible alelles under the control of the nar1 promoter (Brachmann et al., 2001), and its Δcln1 derivate SONU114 were grown in repressive (MM-NH₄) or inductive (MM-NO₃) conditions for b genes expression. Note that both mutant and wild-type cells were able to produce filaments. Bar, 20 μm.