Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1a - Comparison of Predicted Amino Acid Sequences of plant RopGEFs. To identify additional genes encoding DUF315 domain-containing proteins, the amino acid sequence of DUF315 domain was used as a query for BLASTP searches of the NCBI database and the TAIR database. Predicted amino acid sequences of RopGEFs were aligned using ClustalW (www.ebi.ac.uk/clustalw). GenBank accession numbers for various annotated RopGEFs are: Os RopGEF1, AAP54924; Os RopGEF2, AAU10640; Os RopGEF3, BAD28284; Os RopGEF4, XP_506925; Os RopGEF5, NP_915498; Os RopGEF6, NP_916885; Os RopGEF7, XP_450197; Os RopGEF8, XP_463433; Os RopGEF9, XP_473477; Os RopGEF10, XP_475359; Os RopGEF11, Os RopGEF12, XP_478088; Le RopGEF1/KPP, AAW57355.
• Supplemental Figure 1b
• Supplemental Figure 2 - Phylogenetic tree of plant RopGEFs. Data generated from alignment in Supplemental Figure 1 was loaded to Phylodendron Phylogenetic tree printer (iubio.bio.indiana.edu/treeapp) to reconstruct the rooted neighbor-joining phylogenetic tree by the distance-based method. Numbers indicate branching distances.