Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. SOB2 Expression in Wildtype and sob2-D phyB-4 Adult Tissues. Col-0 and sob2-D phyB-4 lines were grown in long day growth conditions (16 h light: 8 h dark). Total RNA was isolated from root (R), mature leaves (ML), young leaves (YL), stems (S), cauline leaves (CL), and flowers (F). SOB2 and UBQ10 cDNA was amplified from total cDNA for 35 cycles. The UBQ10 cDNA was used to normalize each sample.
• Supplemental Figure 2 - Supplemental Figure 2. Phylogenic Analysis of the AP2 Domains of the B-1 Subfamily of AP2 Transcription Factors. A distance neighbor-joining tree of the AP2 domains of the B-1 subfamily of AP2 transcription factors was visualized using PAUP version 4.0b10. The 60 amino acid AP2 domain was aligned using the Gonnet weight matrix of ClustalW. The alignment was not manually adjusted. The tree is rooted with two members (At1g53910 and At3g14230) of the B-2 subfamily of AP2 transcription factors. The tree displays branch lengths and bootstrap values as percentages of 1000 replications.
• Supplemental Figure 3 - Supplemental Figure 3. Alignment of the SOB2/DRN-like and LEP Proteins. Whole protein alignment of SOB2/DRN-like and LEP was performed using the Gonnet weight matrix of ClustalW. The alignment was not manually adjusted. Identical amino acids are in bold black text, while similar amino acids are shaded in red text. The AP2 domain is underlined with a black bar.
• Supplemental Figure 4 - Supplemental Figure 4. Identification of the lep-1 Mutation. (A) Diagram showing the location of the T-DNA insertion in LEP. The AP2 domain is represented in white with black diagonal lines. The locations of the primers used in(B) are depicted by arrows above their location. The red, blue, and green arrows represent the LEP 5’, 5’ T-DNA, and 3’ T-DNA primer sets, respectively. (B) Total RNA was isolated from five-day-old seedlings grown in continuous white light. PCR was performed on cDNA using LEP 5’, 5’ T-DNA, and 3’ T-DNA primers for 35 cycles. The UBQ10 cDNA, amplified for 26 cycles, was used to normalize the amount of cDNA in each of the samples. (C) Four-week-old plants were grown in long day (16 h light/8 h dark) growth conditions.
• Supplemental Figure 5 - Supplemental Figure 5. RGL1 Expression in Wildtype and lep-1 Seeds During Germination. Seeds were incubated as described in Figure 4 for 96h at 4°C, followed by the either 6h, 12h, or 24h at 23°C. cDNA was synthesized from total RNA, and RGL1 cDNA was amplified for 35 cycles. The UBQ10 cDNA amplified for 24 cycles was used to normalize the cDNA templates.