Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Expression of ARF8 and of GUS marker constructs in Ler and arf8-4 plants. (A) and (B) In situ hybridization shows that ARF8 transcripts are present in the carpel mesocarp, the septum and the integuments of developing seed one day post fertilization (A) and three days post fertilization (B) in both Ler and arf8-4. (C) to (F) Expression of P_ARF8:GUS in Ler (C) and arf8-4 (D) flowers at anthesis and in developing siliques of Ler (E) and arf8-4 (F) siliques three days post fertilization showing the same GUS staining pattern. (G) Expression of P_ARF8:GUS in parthenocarpic arf8-4 siliques 7 days post emasculation. (H) Expression of ARF8:GUS was observed from flower stage 5 onwards in arf8-4 plants (I) No expression of ARF8:GUS was detected in arf8-4 seeds at the heart stage of embryo development. Bars =50μm in (A), (B) and (I); 100μm in (E) to (H) and 500μm in (C) and (D). a, anther; cw, carpel wall; dov, degenerating ovule; dpe, days post emasculation; dpf, days post fertilization; em, embryo; fu, funiculus; gy, gynoecium; mc, mesocarp; pd, pedicel; pe, petal; pg, pollen grain; s, seed; se, sepal; sp, septum.
• Supplemental Table 1 - Expression ration of ARF8 mRNA in various tissues.