Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Epistasis analysis between thf1 and gpa1 loss-of-function alleles for sugar sensitivity. Seedlings of gpa1-4, thf1-1 and gpa1-4/thf1-1 backgrounds were germinated on medium supplemented with normal (1%) and elevated (6%) D-glucose. Seven days after germination, both the overall phenotype (panel A) and root length (panel B) were assayed. The gpa1-4/thf1-1 double mutant consistently exhibited the same phenotype as the thf1-1 single mutant, in that it had stunted growth and variegated leaves, and the elongation of the primary root was hypersensitive to elevated D-glucose, compared to gpa1-4.
• Supplemental Movie 1 - Dynamics of stromule interactions with the plasma membrane. Seven-day-old transgenic seedlings expressing THF1-GFP were grown on half MS media supplemented with 1% sugar. Fluorescence images were taken every 15 seconds and saved as a movie sequence with playback of 3 frames per second. See accompanying still image (Figure 3F). The movie highlights the apparent physical but transient interactions between the stomule and plasma membranes.