Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Patterns of the CYCA2;3 Promoter Activity and the Nuclear Localization of the CDKA;1-GFP Fusion Protein in Trichome Development. CYCA2;3promoter activity patterns histochemically analyzed using a CYCA2;3 promoter-GUS reporter gene and the nuclear localization patterns of the CDKA;1-GFP fusion protein in developing trichomes are shown from early (left) to late (right) stages. (A), (B), (G), and (H) Stage 2. (C), (D), (I), and (J) Stage 3. (E) and (K) Stage 4. (F) and (L) Mature trichomes. Bars = 0.05 mm.
• Supplemental Figure 4 - Expression Analysis of ArabidopsisA2-type Cyclin Genes by Real-time Quantitative PCR. Transcripts of Arabidopsis A2-type cyclin genes, CYCA2;1 (triangle),CYCA2;2 (square), CYCA2;3 (filled circle), and CYCA2;4 (diamond), in first leaves (A), cotyledons (B), and etiolated hypocotyls (C) at the indicated time points were quantified by real-time quantitative PCR analysis, and the amounts of the transcripts relative to those of the ACT2 gene were plotted.
• Supplemental Figure 2 - Histochemical Analysis of the CYCA2;2 and CYCA2;4 Promoters. Activities of the CYCA2;2 and CYCA2;4 promoters were histochemically analyzed using promoter-GUS reporter genes introduced into wild-type plants. (A) to (E) GUS-staining patterns of CYCA2;2 promoter-GUS plants in (A) a shoot apex, (B) a young leaf, (C)a cotyledon, (D) a young seedling, and (E) a root apical region. Bars = 1mm. (F) to (I) GUS-staining patterns of CYCA2;4 promoter-GUS plants in (F) a shoot apex, (G) a young seedling, (H) a cotyledon, and (I) roots. Bars = 1 mm.
• Supplemental Figure 3 - Histochemical Analysis of theCYCA2;3 Promoter Activity and Ploidy Distribution Analysis in Etiolated Hypocotyls. (A) CYCA2;3 promoter activity in etiolated seedlings was histochemically analyzed using promoter-GUS reporter genes introduced into wild-type plants. GUS-staining patterns of etiolated seedlings (a) 3, (b) 4, (c) 5, (d) 6, (e) 7, and (f) 8 days after sowing. Bar = 5mm. (B) to (E) GUS-staining patterns of upper parts of etiolated seedlings (B) 5, (C) 6, (D) 7, and (E) 8 days after sowing. Bar = 0.5mm. (F) Ploidy distribution patterns in etiolated hypocotyls of wild-type (WT; upper panels) and CYCA2;3(-/-) (lower panels) plants. Seedlings grown in complete darkness for 11 days after sowing were dissected and subjected to flow cytometry. The ploidy level and the proportion of cells with the level are indicated above each peak.