Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Fluorescence chromatogram of control (grey line) and CHS-silenced hairy root (black line) extracts. Fluorescence excitation was at 365 nm, emission at 450 nm.
• Supplemental Figure 2 - TLC plate of control and CHS-silenced hairy root extracts (not hydrolysed).
• Supplemental Figure 3 - Retention times of standards of liquiritigenin, naringenin, ononin (Formononetin-7-O-β-D-glucopyranoside) and coumestrol. None of these flavonoids could be detected in the root extracts. The insets in the chromatogram for liquiritigenin and naringenin show the detection limit at 10^-8M. All chromatograms show absorbance at 300 nm.
• Supplemental Figure 4 - Schematic setup for auxin transport experiments. Roots of intact hairy root plantlets were treated by placing a small block of NPA (2 x 2 x 5 mm³), or solvent control, or a small drop of rhizobia at the zone of emerging root hairs (“treatment”). After 24 h, the root was cut 5 mm above the point of treatment and a small agar block (2 x 2 x 2 mm³) containing ³H-IAA was positioned at the cut end of the root. The ³H-IAA was allowed to transport acropetally (in the direction of the red arrow) for 12 h. Then the root was cut into two 4 mm long segments, above and below the point of treatment. Each segment was placed in a vial of scintillation fluid and the radioactivity quantified.
• Supplemental Figure 5 - Fluorescence chromatogram of medicarpin standard. Fluorescence excitation was at 365 nm, emission at 450 nm.