Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 2 - BFC clusters. Expression versus time for genes in the 27 circadian BFC clusters. Expression of individual genes (dashed black lines) and cluster average (solid blue line) shown for each cluster. Clock related genes (solid coloured lines; see legends for gene identity) indicated where appropriate. Gene number, Circadian Score (Second Harmonic Ratio, SHR), Amplitude (Second Harmonic Amplitude, SHA) and Phase (Phase by Maximum, PBM) values indicated for each cluster.
• Supplemental Table 1
• Supplemental Table 2
• Supplemental Figure 1 - FLC mRNA expression at 22°C and 27°C. FLC transcript abundance was measured across a circadian cycle by quantitative real time PCR in FRI; FLC genotype seedlings at 22°C (closed bars) or 27°C (open bars). Expression values are the average of two independent biological replicates and normalised to the mean of all samples. Error bars show the range of the two replicates.
• Supplemental Figure 3 - COSOPT and BFC peak time comparisons. Scatter plot of cluster peak phase time for genes in BFC clusters versus COSOPT peak phase time estimates of the individual genes.
• Supplemental Figure 4 - EPR1 leaf movement. Leaf movement period estimates for Col-0 (back bars) and the epr1 T-DNA insertion line SALK_047716 (white bars). Three independent experiments shown at 22°C and two at 27°C. Error bars show variance weighted SEM of period estimates.