Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Analysis of T-DNA Insertion Lines and Their F₁ Progeny (A) RT-PCR analyses of the expression of ATCNGC11 or ATCNGC12 in wt Col-0 plants and homozygous T-DNA insertion lines using specific primers (see Methods). 026568: plants homozygous for a T-DNA insertion in ATCNGC11: 092657 and 092622: plants homozygous for a T-DNA insertion in ATCNGC12. Beta-tubulin was used as a loading control. (B) PCR analysis of T-DNA insertions in F₁ progeny from the following crosses: 092622 x 026568, 092657 x 026568, or 092657 x 026568. F₁ plants were created by reciprocal cross-pollination of three lines. (a) PCR using primers LBb1 and 568RP2 to detect the specific T-DNA insertion in ATCNGC11. (b) PCR using primers LBb1 and 57RP to detect the specific T-DNA insertion in ATCNGC12 (c) PCR using primers LBb1 and 22RP to detect the specific T-DNA insertion in ATCNGC12.
• Supplemental Figure 2 - Morphology in wt Ws plants transformed with ATCNGC11and ATCNGC12 driven by the CaMV 35S promoter. Plants were grown on soil and photographed at 4 wpp.