Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Supplemental Figure 1. RNA Gel Blot Analyses of miR399 Expression under Different Abiotic Stresses. miR399 was detected only in Pi-deficient tissues but not in seedlings subjected to other abiotic stress treatments. C, untreated control; ABA, 100 μM ABA for 3 h; NaCl, 100 mM NaCl for 5 h; PEG, 30% PEG for 5 h; Cold, 4 °C for 10 days, -Pi, Pi-deficient; +Pi, Pi-sufficient. 5S rRNA and tRNA staining is shown as the loading control.
• Supplemental Figure 2 - Supplemental Figure 2. Distribution of Anthocyanin and Pi within Leaves. (A) 8-day-old seedlings were treated under low Pi medium for 12 days. The cotyledons of miR399-overexpressing plants (miR399b, miR399c and miR399f) remained green (blue arrowheads) while those of wild-type plants accumulated large amount of anthocyanin and underwent senescence (red arrowheads). Bar = 0.5 cm. (B) After prolonged Pi starvation treatment in a hydroponic system, the young leaves but not the old leaves of miR399-overexpressing plants started to accumulate anthocyanin. Bar = 1 cm. Distribution of Pi in the leaves of wild-type (Wt, blue triangles) and miR399-overexpressing (miR399b, pink diamonds; miR399c, purple squares; miR399f, red circles) plants grown under low Pi (C) or high Pi (D). Error bars indicate the SD (n= 3).