Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Unrooted Bayesian phylogenetic tree of LOB genes of Arabidopsis, rice and maize, including the ra2 orthologs of rice (Osra2, sorghum (Sbra2)and barley(Hvra2). Grass raorthologs form a monophyletic group and are most closely related toArabidopsis ASL4.Numbers above the branches correspond to the posterior probability for that node. DNA sequences corresponding to the LOB domain were aligned in ClustalX and analyzed with MrBayes.
• Supplemental Figure 2 - Expression of knox genes in wild type and ra2 mutant inflorescences. A, expression of knotted1 by RNA blot hybridization of total RNA of wild type ear (lane 1), ra2-R/ra2-dm ear (lane 2), ra2-Rear (lane 3), wild type tassel (lane 4), and ra2-dmear (lane 5). Top panel shows hybridization with akn1 probe, bottom panel shows the ethidium bromide-stained 26S rRNA band to indicate relative loading. B, reverse-transcription and amplification of gnarly1, liguless3, rough sheath1 and actin from cDNA of B73 inbred line tassels and ears (lanes 1 and 2), ra2-R tassels and ears (lanes 3 and 4), B73 genomic DNA (lane 5), and water control (lane 6).