Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Cycloheximide treatment. (A) In control experiments the effect of cycloheximide on protein synthesis was investigated using strain FB2rGTub1 that contains and additional copy of α-tubulin fused to GFP (GFP-Tub1, (Steinberg et al., 2001) under the control of the induciblecrg-strain. In this strain no GFP-labeled microtubules were detected in glucose-containing medium (A1), but expression of GFP-Tub1 was induced after shift to arabinose-containing medium for at least 2 h (A2). When FB2rGTub1 was grown in arabinose-containing medium for 75 min and cycloheximide was added for additional 45 min protein biosynthesis was blocked and GFP-αTub1 was not synthesized. Bar: 2μm.
• Supplemental Figure 2 - Confrontation assay with yup1^ts mutants. Compatible yup1^ts mutants expressing either GFP or RFP were spotted across from each other and incubated at 34°C.
• Supplemental Figure 3 - Effects of Latrunculin A treatment in Pra1-GFP expressing conjugation hyphae. Conjugation hyphae expressing Pra1-GFP were incubated with Latrunculin A for 90 min thereby enriching Pra1-GFP in the plasma membrane.