Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 (Revised) - Exogenous allyl glucosinolate hydrolysis. Exogenous allyl glucosinolate was added to the glucosinolate hydrolysis assays. The resulting levels of 3,4-epithiobutyl nitrile (EpiNIT) and isothiocyanate (Allyl-ITC) products were assayed via GC-FID. Standard deviation is shown. A. Quantification of the Allyl-ITC outcome of allyl glucosinolate hydrolysis. Six independent plants from each line, Col-0 and Salk-043148 (ESM1 KO), were measured in two independent experiments and the average is shown. Additionally, commercial Myrosinase was utilized to hydrolyze the allyl glucosinolate in the hydrolysis buffer. No significant differences were identified by t-tests within ANOVA combining the data from both experiments. B. Quantification of the EpiNit outcome of allyl glucosinolate hydrolysis. Six independent plants from each line, Col-0 and Salk-043148 (ESM1 KO), were measured in two independent experiments and the average is shown. Additionally, commercial Myrosinase was utilized to hydrolyze the allyl glucosinolate in the hydrolysis buffer. No significant differences were identified by t-tests within ANOVA combining the data from both experiments. C. Allyl glucosinolate was hydrolyzed using Ler leaf extract as a control for hydrolysis in the presence of ESP. The quantification of Allyl-ITC and EpiNit is shown. Six independent plants were measured in two independent experiments and the average is shown. The Allyl-ITC and EpiNit levels are statistically different from all values in section A and B (P < 0.0001, t-test).
• Supplemental Table 1 - Markers utilized for ESM1 fine-scale mapping