Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Expression levels of DPB and E2FC in different organs. (A) Expression levels of DPB in different organs were analyzed by semi-quantitative RT-PCR. F, Flowers; S, Stems; C Caulinar leaves; Rs, Rosette leaves; Sh, 6 day-old shoot; Ro, 6 day-old roots. The actin (ACT2) gene was amplified as a control. (B) Expression levels in different organs/tissues of the E2FC and DPB genes obtained from microarray data available from the GENEVESTIGATOR tool (Zimmermann et al., 2004).
• Supplemental Figure 2 - Overexpression of a truncated ΔE2FC/DPB drastically affects plant development. (A) Phenotype of 6 and 21 day-old wt, ΔE2FC^OE, MYC-DPB^OE and ΔE2FC/MYC-DPB^OE seedlings. Scale bar: for plants of 6 days the bar corresponds to 1 millimeters and for plants of 21 days corresponds to 2 mm. (B) Scanning electron micrographs showing epidermal cells of 4 day-old cotyledons of wt and ΔE2FC/MYC-DPB^OE seedlings. Bar = 200 μm. (C) Ploidy distribution of nuclei extracted from leaves #1/2 of 14 days-old wild type and ΔE2FC/MYC-DPB^OE plants exhibiting a milder phenotype (labeled 1 in panel A). (D) Ploidy distribution of nuclei extracted from whole seedlings (14 day-old) of wild type and ΔE2FC/MYC-DPB^OE plants exhibiting a severe phenotype (labeled 2 in panel A).
• Supplemental Figure 3 - Phenotypes of e2fc-R plants. (A) Scanning electron micrographs of cross sections through mature rosette leaves of 24 days old wt and e2fc-R plants. The micrographs showed the palisade and spongy mesophyll cells and the vascular strands. Bars = 200 μm. (B) Scanning electron micrographs showing epidermal cells of 4 day-old cotyledons of wt and e2fc-R seedlings. Bar = 200 μm. (C) Frequency distribution of epidermal cells with different areas of 4 day-old wt and e2fc-R cotyledons. (D) Ploidy distribution of nuclei extracted from 9 day-old wt and e2fc-R cotyledons.