Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Amino acid sequence alignments of NPP1 with other possible homologs from yellow lupin, Arabidopsis thaliana, barley and tomato.
• Supplemental Figure 10 - Stages to construct pTN1-OsNPP1-GFP plasmid necessary to produce NPP1-GFP expressing green cells of rice.
• Supplemental Figure 2 - Production of NPP1-overexpressing rice plants.
• Supplemental Figure 3 - Wider views of panels “A” and “B” of Figure 5.
• Supplemental Figure 4 - Intracellular immunolocalization of NPPs in leaves using anti-NPP1 polyclonal antiserum.
• Supplemental Figure 5 - Distributions of NPP1 and Rubisco on a density Percoll centrifugation gradient.
• Supplemental Figure 6 - Migrating behavior on SDS-gels of purified ADPG hydrolytic NPP without a previous heat denaturing step.
• Supplemental Figure 7 - Fluorescence images of onion epidermal cells transiently expressing GFP-SYP31 (Golgi marker), GFP-SYP81 (ER marker), GFP (cytosolic marker) and the first 66 and 109 amino-acid residues of NPP1, containing the signal peptide for the ER, fused with GFP.
• Supplemental Figure 8 - Stages to construct pET-OsNPP1 to express rice NPP1 in E. coli.
• Supplemental Figure 9 - Stages to construct p2K-OsNPP1 plasmid necessary to produce NPP1-expressing transgenic rice plants.