Dynamic and Compensatory Responses of Arabidopsis Shoot and Floral Meristems to CLV3 Signaling
Plant Cell Müller et al.
18: 1188
Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1
-
Analysis of cell behaviour. SEM micrographs were taken from meristem replicas every 24 h, and cells were colour coded according to their areal growth rates within this time period (relative size increase, units % h-1). The numbers of cell divisions occuring within 24 h are indicated by dots on individual cells. Asterisks identify the cell at which the geometric center of the SAM surface is located at the beginning of the series. Flower primordia are numbered from the youngest primordium (P0) observed in the sequence to the oldest. Primordium borders as assessed by surface curvatures are outlined in black. Bars = 30 μm. A: Sequence of an untreated control meristem. B-D: Sequences of three independent induced meristems. Replicas were taken at day 0 (B1,D1), 1 (B2,C1,D2), 2 (B3,C2,D3), 3 (C3,D4), 4 (C4) and 6 (C5) after the start of induction.
Supplemental Figure 2
-
Meristem size measurements. The sizes of shoot apical meristems were determined in cleared seedlings. In median optical sections, the surfaces of meristems were measured along the indicated red lines. For better identification, L1 cells were outlined. A: clv3-2 mutant seedlings, carrying the pCLV3 transgene. B: clv3-2 mutant.