Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Comparison of germination frequency between wild-type (Columbia) and KnockOLEO1 plants. Arabidopsis seeds were germinated in wet filter paper in the presence of light.
• Supplemental Figure 2 - Comparison of seedling growth. Arabidopsis seeds were germinated in half strength MS media without sucrose supplement. (A) and (B) Five days old wild-type and SupOLEO1-Loop seedlings, respectively.
• Supplemental Figure 3 - Confocal section of Arabidopsis SupOLEO1 seedlings 8 days after germination. Seeds were germinated in half strength MS media without sucrose supplement, stained with Nile red and analysed by confocal microscopy. Autofluorescence of chlorophyll was detected in the blue channel.
• Supplemental Figure 4 - Suppression of OLEO1 in Arabidopsis using different constructs. Oilbodies were extracted from T2 seeds and submitted to SDS-PAGE. Each lane contains oilbody-associated proteins obtained from individual transgenic events. The events displaying significant reduction of OLEO1 in comparison to OLEO2 and OLEO4 are indicated by arrows. (A), (B) and (C) are transgenic events obtained using Antisense, Hairpin and Loop cassettes, respectively.
• Supplemental Figure 5 - Thin layer chromatography of oilbody lipids. Oilbodies were isolated from different plants and total lipids were extracted from these organelles. Lipids were applied on silica Gel 60 F254 plates and half-developed with chloroform-methanol-acetic acid-formic acid-water (70:30:12:4:2 v/v) and fully developed with hexane-diethyl ether-acetic acid (65:35:2 v/v). The lipids were visualized by heating the plates after they have been dipped in a solution containing cupric acetate (3%) and phosphoric acid (8%). Abbreviations are PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositol (PI) and TAG, triacylglycerol.