Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Genomic Organization of jekyll in Barley and Other Plants. (A) jekyl-like sequences in different species. Genomic DNA (10 μg per line) was digested with Bam HI, fractionated on a 1% agarose gel, and hybridized with the jekyll probe. The size of standard markers given in kb (right). Hv, Hordeum vulgare; Ta, Triticum aestivum; Zm, Zea mays; Po, Pennisetum orientale; Os, Oryza sativa; Sc, Secale cereale; As, Avena sativa; At, Arabidopsis thaliana; Nt, Nicotiana tabacum. (B) Phylogenetic relationships of species sampled. Representatives of subfamily Triticeae are shown in bold. (C) Amino acid alignment of JEKYLL (Jek) with predicted proteins from Triticum aestivum (TJ1 and TJ2). Residues shared by at least two sequences are shaded gray. Consensus sequence (Cons.) is shown below in bold.
• Supplemental Figure 2 - The Influence of JEKYLL Domains on Growth of E. coli Cells. Full length and truncated sequences of JEKYLL were cloned into pET23a (schematic representations of the constructs pJ1-pJ6 are shown in insert) and transformed into E. coli cells. E. coli cultures were grown in the presence of IPTG as an activator of protein expression, and optical densities (600 nm) of the bacterial cultures were measured every hour.
• Supplemental Figure 3 - Influence of jekyll on Phenotype of Transgenic Tobacco Plants Seven Days after Induction of the Gene Expression. Wild type and transgenic tobacco plants transformed with the construct pAlc-jekyll were grown in pots in greenhouse. jekyll expression was activated by watering with 2% ethanol during three days. Transgenic plants showed loss of turgor after approximately 24 h of first watering and subsequent growth delay. Expression of jekyll after induction with ethanol was checked by northern blots (inset).