Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Small RNA and mRNA expression of repeat-containing genes and transposons in ago1-9. Small RNA (left panels) and RT-PCR (top right panels) profiles of HPL1 (A), At2g13820 (B) and a VANDAL14 element (C) in wild-type (Ler) and ago1-9 RNA. Regions of each gene amplified by RT-PCR are indicated by black bars (A–C); regions corresponding to sense-strand riboprobes are indicated in gray (A–B) or black (C). Small RNA blots were loaded with 20 μg PEG-precipitated total RNA. All blots were also hybridized with probes corresponding to miR171 and U6 snRNA as controls. Sizes are indicated to the left of each panel in nucleotides, with the exception of top left panels (indicated in kilobases). RNAs used for RT-PCR were identical to those in Figure 5.
• Supplemental Table 3 - miRNA Targets and Genes Showing Consistently Altered mRNA Levels in ago1 and dcl.
• Supplemental Table 1 - Overview of transcript levels in wild-type, ago1 and dcl1.
• Supplemental Table 2 - Gene Ontology classification of transcripts that change significantly in ago1 and dcl1.
• Supplemental Table 4 - Fold changes of features that change significantly in expression in ago1 and dcl1.
• Supplemental Table 5 - Positive and negative controls for the AtGenome1 microarray.
• Supplemental Table 6 - Primer sequences for Figures 1–5.