Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - PVX:GFP resistance assay with Rx variants. Rx variant were expressed via agro-infiltration under the control of P_Rx (A) with an infectious PVX:GFP clone. After five days the infected leaf patch was analyzed for GFP fluorescence. Note that GFP fluorescence was observed prior to the onset of any HR in cases where an HR was initiated by partially active Rx variants after approximately seven days. Similar experiments were performed with Rx and chimaeric derivatives (B) except that expression was driven from P_35S.
• Supplemental Figure 2 - Comprehensive list of results from functional assays performed with Rx/GPA2 chimaeras generated during the course of these experiments. An asterix indicates that the inactivating P-loop mutation (GK175AA) was introduced into the corresponding clones when co-expressed with CC-NB-ARC. + indicates that a strong HR was observed two days after infiltration and - indicates no HR. -/+ indicates that an HR was observed but was consistently delayed by ˜24hrs compared to wild-type Rx plus CP. Each experiment was repeated at least twice with similar results. na; not applicable, nd; not done
• Supplemental Figure 3 - Stable expression of non-transactivating proteins. The indicated constructs were transiently expressed via agro-infiltration under the control of P_35S. Two days post-infiltration protein extracts were immunoblotted with αHA-antibody. This experiment was repeated twice, with similar results.