Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - In vitro titration curves for FLIPglu-170nΔ13, FLIPglu-2μΔ13. FLIPglu-600μΔ13 and FLIPglu-3.2mΔ13. The graph shows absolute values.
• Supplemental Figure 2 - In vitro eYFP/eCFP ratio for FLIPglu-600μΔ13 at pH 7.0 and .5. Titrations were done with 2μM and 20mM glucose in 20mM MES/Tris buffer.
• Supplemental Figure 3 - DNA sequence alignment of the synthetic genes Ares, Aphrodite with eCFP and eYFP.
• Supplemental Table 1 - Details of the nanosensors used for Arabidopsis transformations which showed silencing patterns similar to those observed with FLIPgluΔ13.
• Supplemental Movie 1 - Time lapse movie derived from the experiment shown in Figure 4. The FRET sensor FLIPglu-600μΔ13 with an affinity of 600μM for glucose in the stably transformed rdr6-11 Arabidopsis plants responds in the epidermis to perfusion with 50mM glucose.