Supplemental Data

Files in this Data Supplement:

• Supplemental Movie 1 - Simultaneous visual and sonic representation of pH changes and growth rate in a lily pollen tube. Integrating visual and sonic data sets provides a clear representation of the temporal relationship of pH to pollen tube growth velocity. The three percussion sounds represent data from the cell in Figure 1. The first sound (resembles a saw), which represents maximal pH, is also analogous to high ADF activity in our model. It symbolizes the fragmentation of actin filaments, which leads to more actin polymerization and faster growth rates. Maximal growth rate is the second percussion sound (resembles a heart beat), which is then followed by the last percussion sound, minimal pH, symbolizing ADF/AIP inactivation and slower growth rates. The combined visual and sound effects emphasize the fact that each peak of growth rate is preceded by a high pH and followed by an acidic wave.