Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Lineweaver-Burke plot showing apparent K_m for Thr of THA2
• Supplemental Figure 10 - Photographs of tha2-1/tha2-1 P_35S-omr1-5 rosette and flowers
• Supplemental Figure 11 - Seed amino acid changes resulting from seed-specific expression of P_35S-omr1-5 in the tha2/tha2 and tha1/tha1 genetic backgrounds
• Supplemental Figure 12 - DNA sequence comparison of Thr aldolases from Arabidopsis and selected crop plants
• Supplemental Table 1 - Sequences of the junction sites of THA1 and THA2 T-DNA insertion lines.
• Supplemental Table 2 - PCR primers used in the experiments.
• Supplemental Table 3 - GC-MS metabolite profiling of threonine aldolase mutants
• Supplemental Figure 2 - Growth of yeast expressing Arabidopsis tha1-1 on medium containing 50 μM Gly
• Supplemental Figure 3 - PCR to confirm zygosity and RT-PCR to confirm gene expression of Thr aldolase mutants
• Supplemental Figure 4 - Complementation of the tha1-1 amino acid phenotype by transformation
• Supplemental Figure 5 - Thr levels in single seeds of THA1/tha1 heterozygotes
• Supplemental Figure 6 - Thr IC₅₀ determination for wild type, tha1, and P_35S-omr1 seedlings
• Supplemental Figure 7 - Chlorophyll content of tha2 mutant seeds and seedlings
• Supplemental Figure 8 - Amino acid content of tha1 THA2/tha2 double mutants
• Supplemental Figure 9 - Digital Northern blot of THA1 and THA2 expression in Arabidopsis