Supplemental Data

Files in this Data Supplement:

• Supplemental Table 1 - Percent C. heterostrophus spore germination, in vitro.
• Supplemental Figure 6 - Confirmation of integration of NcNPS6.
• Supplemental Figure 7 - Structures of deferriated coprogens depicting cleavages that explain fragment ions at m/z 285 and 397 that were observed in ESI-MS-MS spectra of coprogen and isotriornicin.
• Supplemental Figure 8 - The NRPSs encoded by F.graminearum NPS6 and A. brassicicola NPS6 are responsible for biosynthesis of extracellular siderophores.
• Supplemental Figure 9 - Survival curves of wheat spikes infected by WT and a Δnps6 strain of F. graminearum with or without exogenous application of iron.
• Supplemental Figure 10 - Increased sensitivity of the Δnps6 strain of A. brassicicola to iron depletion generated by 2DP and restoration of tolerance to iron depletion by in vitro application of DFO.
• Supplemental Figure 11 - Restoration of tolerance to ROS by application of ferric citrate or DFO.
• Supplemental Methods
• Supplemental Table 2 - PCR diagnosis of type of integration of transforming DNA.
• Supplemental Table 3 - Quantitative analysis of C. heterostrophus intra- and extra-cellular siderophore production.
• Supplemental Table 4 - Strains used in this study.
• Supplemental Figure 1 - C. heterostrophus Δnps6 strain shows reduced virulence to corn and hypersensitivity to H₂O₂.
• Supplemental Figure 2 - The Δnps6 strain of C. heterostrophus shows a defect in colonization in planta.
• Supplemental Figure 3 - Strategy for and confirmation of gene deletion.
• Supplemental Figure 4 - Survival curves of wheat spikes challenged by WT and two Δnps6 strains of F. graminearum.
• Supplemental Figure 5 - Hypersensitivity of Δnps6 strains of A. brassicicola and F. graminearum to iron depletion.