Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - Morphological comparison of WT and ise2 embryos revealing the enlarged SAM region in ise2.
• Supplemental Figure 2 - Comparison of consensus DEVH RNA helicase motifs to such motifs in ISE2 and Ski2P from yeast, and phylogenetic tree of ISE2 homologs in diverse organisms.
• Supplemental Figure 3 - The embryo-lethal ise2-2 phenotype was fully rescued by transformation with the ISE2-GFP sequence.
• Supplemental Figure 4 - ISE2 expression analysis.
• Supplemental Figure 5 - Vein phenotypes caused by the ise2 mutation.
• Supplemental Figure 6 - Frequency of ISE2-granules in control and heat-stressed samples.